| 产品名称 | Pre-Pulled Microcapillary Needles (10/pack) |
| 产品货号 | |
| 产品价格 | 现货询价，电话：010-67529703 |
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| 产品品牌 | Tritech ResearchInc |
| 产品概述 | Pre-Pulled Microcapillary Needles (10/pack) |
| 产品详情 |
 (Click to see larger image) Tritech Research offers high-quality pre-pulled microcapillary needles suitable for injection of C. elegans, Drosophila, Zebrafish, Xenopus, mammalian cells, or any other application requiring fine tipped injection needles. Our pre-pulled injection needles have an outside diameter of 1.0mm O.D. and an inside diameter of 0.6mm I.D. along the shaft. The actual tip diameter of our microinjection needles is less than 1 micron (< 1 µm). We offer the needles with two options: containing an internal glass filament for easy back-loading, and without. The option with filament is super convenient for applications requiring less than 1 microliter in the tip. The back of the needle can be placed in a microcentrifuge tube with a drop of injection solution (for example DNA) in it, and the solution will climb up the interface between the filament and the ID of the microcapillary tube. Unless you watch with a microscope, it seems like magic as the solution starts filling from the tip backwards (after 1-5 minutes of the solution climbing up the fllament)! The option without filament may be more convenient for application where more than 1 µl is backloaded into the needle using a loading needle. Both types are available in packs of 10. If you are interested in other sizes/types of pre-pulled needles, please contact our sales department.
FAQ's Q: How do I open the tip of a pre-pulled needle without compromising the sharpness of the needle or using razor blades to cut the tip? A: You can follow this simple procedure to etch the tip of your borosilicate microcapillary needle with hydrofluoric acid (HF): 1) Firmly secure your pre-pulled needle in the MINJ-4 needle holder attached to your MINJ-D (or other pressure driven microinjector.) 2) Adjust the MINJ-D to the following settings: Time= Manual (0.0 sec); injP=70 kPa; holdP=0 kPa; aspiration thumbscrew should be set fully clockwise; 3) Place a 200-microliter drop of concentrated HF into a lid from a 100ml plastic (not glass as the acid will damage or eat through it) Petri dish and place it under your dissecting scope. 4) Have a 500 mL beaker (or similar vessel) half-full of water nearby. 5) Begin an injection with the microinjector (by holding down the foot pedal) and carefully stick the needle tip into the acid drop. 6) The acid will begin etching away the tip of the needle. As soon as you see bubbles coming from the needle tip, remove it from the acid and place it into the beaker of water to dilute/neutralize/stop the acid reaction. 7) After removing the needle from the water, stop the injection by releasing the foot pedal. You should now have a really fine, sharp open needle. |
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