Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.
| Target |
RAF1 |
| Reactivity |
Human |
| Host |
Mouse |
| Clonality |
Monoclonal |
| Tested Applications |
ELISA, WB, IHC, FCM |
| Recommended dilutions |
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, FCM: 1/200 - 1/400. Optimal dilutions/concentrations should be determined by the end user. |
| Immunogen |
Purified recombinant fragment of human RAF1 expressed in E. Coli. |
| Purification |
Purified antibody |
| Isotype |
IgG1 |
| Conjugation |
Unconjugated |
| Storage |
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Molecular Weight |
72 kDa |
| Swiss Prot |
P04049
|
| GeneID |
5894
|
| Gene Symbol |
RAF1 |
| OMIM |
164760
|
| Enzyme Commission Number |
EC 2.7.11.1 |
| HGNC |
9829 |
| Ensembl |
ENSG00000132155 |
| Concentration |
1.0 mg/ml |
| Buffer |
PBS with 0.05% sodium azide and 0.5% protein stabilizer. |
| UNSPSC Code |
12352203 |
| Availability |
Shipped within 5-10 working days. |
| Note |
This product is for research use only. |