RAD17 Antibody is a Rabbit Polyclonal antibody against RAD17. The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified.

Target	RAD17
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Tested Applications	WB, IF/ICC, IP
Recommended dilutions	WB: 1/500 - 1/2000, IF/ICC: 1/10 - 1/100, IP: 1/50 - 1/200. Optimal dilutions/concentrations should be determined by the end user.
Immunogen	Recombinant protein of human RAD17.
Purification	Affinity purified.
Form	Liquid
Isotype	IgG
Conjugation	Unconjugated
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Molecular Weight	Calculated MW: 57 kDa/66 kDa/75 kDa/77 kDa Observed MW: 85 kDa
Swiss Prot	O75943
GeneID	5884
Gene Symbol	RAD17
Concentration	> 1 mg/ml
Buffer	PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
UNSPSC Code	12352203
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.