Rabbit polyclonal antibody against Akt protein. Immunogen region is C-terminal. Specificity is as follows for the reactive species: H:S473, M:S473, R:S473.
| Target |
Akt |
| Reactivity |
Human, Mouse, Rat |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Tested Applications |
WB, IHC, IF/ICC |
| Recommended dilutions |
WB: 1/500 - 1/3000, IHC: 1/50 - 1/200, IF/ICC: 1/100 - 1/500. Optimal dilutions/concentrations should be determined by the end user. |
| Immunogen |
The antiserum was produced against synthesized phosphopeptide derived from human Akt around the phosphorylation site of Serine 473 (Q-F-SP-Y-S). |
| Purification |
Purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen. |
| Form |
Liquid |
| Isotype |
IgG |
| Conjugation |
Unconjugated |
| Specificity |
Detects endogenous levels of Akt only when phosphorylated at Ser473. |
| Storage |
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Swiss Prot |
P31749
|
| Enzyme Commission Number |
EC 2.7.11.1 |
| Concentration |
1 mg/ml |
| Buffer |
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide, 50% glycerol. |
| UNSPSC Code |
12352203 |
| Availability |
Shipped within 5-10 working days. |
| Note |
This product is for research use only. |