Dual specificity protein tyrosine phosphatases (dsPTPs) are a subfamily of protein tyrosine phosphatases implicated in the regulation of mitogen-activated protein kinase (MAPK). MKP-1 (also known as CL100, 3CH134, Erp, and hVH-1) exemplifies a class of dual-specificity phosphatase able to reverse the activation of MAP kinases by dephosphorylating critical tyrosine and threonine residues. MKP-3 is 36% identical to MKP-1 and it blocks both the phosphorylation and enzymatic activation of ERK2 by mitogens. MKP-3 mRNA is expressed in lung, heart, brain, and kidney, but not significantly in skeletal muscle or testis.
Target |
MKP3 |
Reactivity |
Rat |
Host |
Rabbit |
Clonality |
Polyclonal |
Tested Applications |
WB, FCM |
Recommended dilutions |
Optimal dilutions/concentrations should be determined by the end user. |
Immunogen |
6x HIS-tagged recombinant protein encoding 1-381 AA of rat MKP3. |
Purification |
Purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
Isotype |
IgG |
Conjugation |
Unconjugated |
Storage |
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
Swiss Prot |
Q64346
|
NCBI Accession |
NP_446335.1
|
Buffer |
PBS containing 0.09% sodium azide. |
UNSPSC Code |
12352203 |
Availability |
Shipped within 5-10 working days. |
Note |
This product is for research use only. |