MDM4, encodes a 490-amino acid protein containing a RING finger domain and a putative nuclear localization signal.The MDM4 putative nuclear localization signal, which all Mdm proteins contain, is located in the C-terminal region of the protein.The mRNA is expressed at a high level in thymus and at lower levels in all other tissues tested.MDM4 protein produced by in vitro translation interacts with p53 via a binding domain located in the N-terminal region of the MDM4 protein.MDM4 shows significant structural similarity to p53-binding protein MDM2.
| Target |
MDM4 |
| Reactivity |
Human |
| Host |
Mouse |
| Clonality |
Monoclonal |
| Tested Applications |
ELISA, WB, IHC, IF/ICC |
| Recommended dilutions |
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, IF/ICC: 1/200 - 1/1000. Optimal dilutions/concentrations should be determined by the end user. |
| Immunogen |
Purified recombinant fragment of human MDM4 expressed in E. coli. |
| Purification |
Unpurified Ascites. |
| Isotype |
IgG1 |
| Conjugation |
Unconjugated |
| Storage |
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Molecular Weight |
55 kDa |
| Swiss Prot |
O15151
|
| GeneID |
4194
|
| Gene Symbol |
MDM4 |
| OMIM |
602704
|
| HGNC |
6974 |
| Ensembl |
ENSG00000198625 |
| Buffer |
Ascitic fluid containing 0.03% sodium azide. |
| UNSPSC Code |
12352203 |
| Availability |
Shipped within 5-10 working days. |
| Note |
This product is for research use only. |