The CD15 antigen is expressed on approximately 90% human circulating granulocytes (membranes and granules), 30 to 60% of circulating monocytes and is absent from normal lymphocytes. CD15 antigen is also expressed on Reed Sternberg cells of Hodgkin's disease, on T cell lymphomas including mycosis fungoides and on some leukemias. Outside the hematopoietic system, CD15 expression is described in certain normal and neoplastic epithelial cells and in astrocytes. At least 5 major CD15 antigens (105, 135, 165, 185, 220 kDa) are present on the surface membranes of polymorphonuclear cells.
| Target |
CD15 |
| Reactivity |
Human |
| Host |
Mouse |
| Clonality |
Monoclonal |
| Tested Applications |
FCM |
| Recommended dilutions |
FCM: 20 µl/1 million cells. Optimal dilutions/concentrations should be determined by the end user. |
| Immunogen |
X-hapten on lacto-N-fucose pentaosyl lll. |
| Purification |
Affinity Chromatography |
| Form |
Liquid |
| Isotype |
IgG3 |
| Conjugation |
Biotin |
| Specificity |
The CD15 antigen is the X-hapten on lacto-N-fucose pentaosyl lll, which is expressed on circulating granulocytes or tissue granulocytes as well as neutrophils and eosinophils. The CD15 antibody recognizes the carbohydrate structure 3-fucosyl-N-acetyl-lactosamine. It specifically labels neutrophils, eosinophils and monoblastoid precursor cells of the myeloid lineage. |
| Storage |
Aliquot and store in the dark at 2°C to 8°C. Avoid exposure to light. |
| Molecular Weight |
45 kDa |
| Swiss Prot |
P22084
|
| GeneID |
2526
|
| Buffer |
The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium Azide |
| UNSPSC Code |
12352203 |
| Availability |
Shipped within 3-5 working days. |
| Note |
This product is for research use only. |