| 产品名称 | NxGen™ T4 DNA Ligase, Lucigen |
| 产品货号 | 30241-1 |
| 产品价格 | 现货询价，电话：010-67529703 |
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| 产品品牌 | vwr |
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Lucigen’s NxGen™ T4 DNA Ligase reliably joins double-stranded nucleic acid chains.
T4 DNA Ligase catalyzes and joins strands of dsRNA and dsDNA by forming phosphodiester bonds. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA, or DNA/RNA hybrids.
10X T4 DNA Ligase Buffer is composed of 500mM Tris-HCI, 100 mM MgCl2, 50 mM dithiothreitol, 10 mM ATP, pH 7.6 @ 25°C. 2X Rapid Ligation Buffer is composed of 132mM Tris-HCI, 20 mM MgCl2, 2 mM dithiothreitol, 2 mM ATP, 15% PEG, pH 7.6 @ 25°C.
Performance Specifications: 1) SS Exonuclease: 6,000 U <0.1% released. 2) DS Exonuclease: 6,000 U <0.1% released. 3) Endonuclease: 6,000 U <0.1% converted. 4) E. coli 16S rDNA Contamination: 3,000 U < 10 copies. 5) Storage: -20°C.
Source: A recombinant E. coli strain carrying the cloned T4 DNA Ligase gene.
Ordering information: Enzyme is provided in 10 mM Tris-HCl, 50 mM NaCl, 1 mM dithiothreitol, 0.1 mM EDTA, 50% glycerol, pH 7.5 at 25°C. Unit definition: 1 unit is defined as the amount of T4 DNA Ligase required to join 50% of 100 ng of DNA fragments with cohesive termini in 50 µl 1 X T4 DNA Ligase Buffer following a 30 minute incubation at 23°C. Note: This product is sold for in vitro research use only under license from Brookhaven National Laboratory, U.S. Patent Nos. 4,952,496; 5,693,489; and 5,869,320.
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