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| Product Name | HeLa Cell Lysate, Heat Shocked |
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| Description | HeLa Cell Lysate (Heat Shocked) is useful in immunoblotting applications as a positive control for many antibodies, particularly those recognizing proteins induced by heat shock. The cells are grown, heat shocked for 2 hours at 44°C, and allowed to recover at 37°C for 5 to18 hours before harvest. The cells are then washed, lysed, sonicated, and resuspended in SDS-PAGE sample buffer. The HeLa cell line was originally derived from epithelioid carcinoma tissue (cervix) from a human donor. Research into the heat shock response provides a model for the study of the effects of environmental and homeostatic stressors upon cells. Heat shocking reproducibly alters gene expression in cells resulting in the subsequent production of a variety of morphological changes as well as the expression of heat shock proteins which are crucial for the maintenance of cellular homeostasis during normal cell growth and for survival during and after various cellular stresses. Heat shocking has been shown to incr |
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| Size | 200ug |
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| Concentration | n/a |
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| Applications | WB |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | H1838-01 |
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| Order / More Info | HeLa Cell Lysate, Heat Shocked from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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