| Description | Hypoxia Inducible Factor 1 Alpha (HIF1a) BioAssay™ ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of HIF1a in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Detection Range: 0.156-10ng/ml Sensitivity: 0.061ng/ml Precision: Intra-Assay %CV: <10% Inter-Assay %CV: <12% Kit Components: *025741A: Microtiter Plate, 1x48 wells or 1x96 wells, Pre-coated; ready to use. *025741B: Standard, 2x1vial 025741C: Standard Diluent, 1x10ml or 1x20ml *025741D: Detection Reagent A, 1x60ul or 1x120ul *025741E: Detection Reagent B, 1x60ul or 1x120ul 025741F: Assay Diluent A, 1x6ml or 1x12ml 025741G: Assay Diluent B, 1x6ml or 1x12ml 025741H: TMB Substrate, 1x4.5ml or 1x9ml *025741J: Positive Control, 1.05ng/ml, 1x1vial 025741K: Stop Solution, 1x3ml or 1x6ml 025741L: Wash Buffer, 30X, 1x10ml or 1x20ml Precaution: The Stop Solution (025741K) suggested for use with this kit is an acid solution. Wear eye, hand |
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