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| Product Name | Alanine Aminotraasferase (ALT/GPT) BioAssay™ Kit |
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| Description | Sample Type: Serum, plasma, tissue, culture cells and cell culture fluid Intended Use: This kit can be used for quantitative determination of NEFA in serum, plasma, tissue homogenate, cells (or cell supernatant). Test Principle: Glutamic-pyruvic transaminase (Alanine aminotransferase /ALT /GPT) can react with the substrate consisted of alanine and alpha-ketoglutaric acid in pH7.4 solution at 37°C, and then produce alanine and glutamic acid. Add 2,4-dinitrophenylhydrazine (DNPH) hydrochloric acid solution after 30 min to stop the reaction. Then the addition reaction occurs between DNPH and the carbonyl in ketonic acid and produce pyruvic acid hydrazine. The phenylhydrazone can show brown color in alkaline condition. Measure the OD values at 505nm and calculate the enzyme activity. |
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| Size | 1 kit |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 405739 |
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| Order / More Info | Alanine Aminotraasferase (ALT/GPT) BioAssay™ Kit from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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