| 产品详情 |
| Edit |   |
|---|
| Product Name | Active Recombinant Human Interleukin-3 |
|---|
| Description | Human recombinant protein expressed in Nicotiana benthamiana. It is produced by transient expression in non-transgenic plants. This product contains no animal-derived components or impurities. Animal Free product. Interleukin-3 is a potent growth factor involved in a variety of cell activities such as cell growth, differentiation and apoptosis. It takes part of several biological responses such as proliferation, and differentiation of a broad range of hematopoietic progenitor cells into erythrocytes, granulocytes, monocytes, megakaryocytic and mast cells. IL-3 also induces the production of several enzymes involved in cellular metabolism, differentiation, and DNA/RNA metabolism. IL-3 is produced by activated T-lymphocytes, keratinocytes, NK-cells, mast cells, endothelial cells and monocytes. The biological activity of IL-3 is mediated through specific cell surface receptor that is composed of alpha and beta subunits. Alpha subunit is responsible for the binding and beta subunit transmits signals across the plasma membrane; il-3 is known to activate three signalling pathways: JAK/STAT, RAS/RAF/MAP kinase, and the PI-3kinase/PKB pathways. IL-3 is also implicated in the pathogenesis of several diseases such as asthma, athero sclerosis and multiple sclerosis. Recombinant protein has been widely used in clinical practice, in the treatment of leukaemia and as therapy for patients with bone marrow deficiency function. Recombinant human Interleukin-3 is a glycosylated polypeptide chain containing 133 amino acids (20 - 152 of P08700 IL3_HUMAN) and a His-tag at the N-terminal end. It has a predicted molecular mass of 16.2 KDa, however as result of glycosylation, the recombinant protein could migrate with an apparent molecular mass of 18-22 kDa in SDSPAGE. Purity >97% by SDS-PAGE gel |
|---|
| Size | 1 µg, 10 µg, 20 µg, 100 µg |
|---|
| Concentration | n/a |
|---|
| Applications | Functional studies, Cell culture, Western Blot |
|---|
| Other Names | MCGF / MULTI-CSF |
|---|
| Gene, Accession, CAS # | UniProt: P08700 |
|---|
| Catalog # | RF0042 |
|---|
| Price | |
|---|
| Order / More Info | Active Recombinant Human Interleukin-3 from AGRENVEC |
|---|
| Product Specific References | - Schroeder, J. T. et al., 2009. J. Immunol. 182 2432-2438. - Asquith, K et al., 2008. J. Immunol. 180 1199-1206. - Munitz, A. et al., 2006. J. Immunol. 177 77-83. - Robin, C. et al., 2006. An unexpected role for IL-3 in the embryonic development of hematopoietic stem cells. Dev Cell., Aug; 11(2): 6097-60104. - Starr, R. et al., 1997. A family of cytokine -inducible inhibitors of signalling. Nature 387 917-921. - Agglietta, M. et al., 1993. Interleukin-3 in vivo: kinetic of response of target cells. Blood 82: 2054-2061. - Kitamura, T. et al., 1991. Expression cloning of the human IL-3 receptor cDNA revels a shared beta subunit for the human IL-3 and GMCSF receptors. Cell 66 1165-1174. ? |
|---|
| 产品资料 |
|
|