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| Product Name | Active Recombinant Human Interleukin-4 |
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| Description | Human recombinant protein expressed in Nicotiana benthamiana. It is produced by transient expression in non-transgenic plants. This product contains no animal-derived components or impurities. Animal Free product. Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses including differentiation of naive T cells into the TH2 phenotype, promoting B cell proliferation, antibody isotype switching, and expression of other TH2 cytokines including IL-5 and IL-9. IL-4 plays a critical role in the development of allergic inflammation and asthma.It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. IL-4 is produced primarily by activated CD4+ T-lymphocytes, NK 1.1+ T-cells (NKT), mast cells and basophils. One major role of IL-4 is priming the differentiation of precursor CD4+ T-cells into Th2 effector cells by altering their cytokine expression during activation. IL-4 has also been shown to have other stimulatory effects on B-cells including increased expression of CD23, IL-4R, IgM and MHC class II molecules on their surface. Recombinant human Interleukin-4 is a polypeptide chain containing 137 amino acids (25 - 153 of P05112 IL4_HUMAN) and 8 aa Histidine-based tag. It has a predicted molecular mass of 16kDa, however as result of potential glycosylation, the recombinant protein could migrate with an apparent molecular mass of 16-18 kDa in SDS-PAGE gel. Purity >97% by SDS-PAGE gel |
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| Size | 1 µg, 10 µg, 50 µg, 100 µg,250 µg |
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| Concentration | n/a |
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| Applications | Functional studies, Cell assay, SDS-PAGE, Western Blot, Antibody Production |
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| Other Names | B-cell stimulatory factor 1 |
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| Gene, Accession, CAS # | UniProt: P05112 |
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| Catalog # | RF0099 |
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| Order / More Info | Active Recombinant Human Interleukin-4 from AGRENVEC |
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| Product Specific References | - Kitamura, T. et al., 1989. Establishment and characterization of a unique human cell line that proliferates dependently on GMCSF, IL-3 or erythropoietin. J. Cell Phyisiol., 140 (3 ): 323-334. - Paul, W.E., 1991.Interleukin-4: a prototypic immunoregulatory lymphokine. Blood, 77, 1859-1870. - Callard, R.E. et al., 1996. IL-4 and IL-13 receptors: are they one and the same? Immunol. Today, 17, 108-110. - O Garra, A., and Arai, N., 2000.The molecular basis of T helper 1 and T helper 2 cell differentiation. Trends Cell Biol., 10, 542-550. - Ryan, J.J. et al. 2007. Mast cell homeostasis: a fundamental aspect of allergic disease. Crit. Rev. Immunol., 27, 15-32. |
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