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| Product Name | PCR Cloning BioGenomics™ Kit |
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| Description | Description: The PCR Cloning Kit is a simple and highly efficient method to insert any gene or DNA fragment into a vector, without time-consuming ligation steps. • No ligation steps required • Multiple PCR inserts can be added in a single step • Efficiencies of > 90% are typical. • Go from PCR product to transforming cells in under an hour • Suitable for fixing or adding point mutations, insertions or deletions. Test Principle: A primer set homologous to the gene of interest and the vector is used to amplify a PCR product. This purified PCR product is incubated with restriction enzyme-digested vector and Reagent A of the PCR Cloning BioAssay™ Kit. Following a brief incubation with Reagent B, the annealed vector is ready for transformation. Primer Design: Proper primer design is critical for successful PCR and transformation steps. Primers for the gene of interest should contain the restriction site as well as at least 10 nucleotides of sequence homologous |
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| Size | 10 tests |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 137318 |
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| Order / More Info | PCR Cloning BioGenomics™ Kit from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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