| 产品详情 |
| Edit |   |
|---|
| Antigenic Specificity | GEF-H1, Human |
|---|
| Clone | B4/7 |
|---|
| Host Species | Mouse |
|---|
| Reactive Species | human, canine |
|---|
| Isotype | IgG1 |
|---|
| Format | unconjugated |
|---|
| Size | 100 µg |
|---|
| Concentration | 100 µg/ml |
|---|
| Applications | frozen sections, IF, IP, WB |
|---|
| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
|---|
| Description | The monoclonal antibody B4/7 recognizes human and canine guanine nucleotide exchange factor H1 (GEF-H1). GEFH1 is an ~110 kDa protein belonging to the Dbl family of proto-oncogenes. GEF-H1 can activate the small GTPase RhoA, but not Rac1 or Cdc42. Rho family GTPases are central regulators of epithelial tight junctions and the cytoskeleton. GEF-H1 can associate with different cytoskeletal structures, namely microtubules and the actin cytoskeleton. It has also been proposed to mediate crosstalk between the two types of filaments. Localization of GEF-H1 differs between cell types. In MRC-5 fibroblast cells, GEF-H1 localizes to stress fibers. In epithelial cells, GEF-H1 is associated with apical tight junctions and involved in regulating paracellular permeability of small hydrophilic tracers. Furthermore, its subcellular localization changes in mitotic cells, where endogenous GEF-H1 is concentrated at mitotic spindles. GEF-H1 is capable of binding to the F-actin binding junctional adaptor cingulin. Binding to cingulin inhibits GEF-H1 and results in the downregulation of RhoA and inhibition of G1/S phase transition. In low confluent cultured cells, the localization of GEF-H1 is predominantly cytoplasmic. With increasing density of the cells, free GEF-H1 is sequestered at the tight junctions by cingulin. GEF-H1 is part of the signaling pathway connecting epithelial polarity with the cell cycle, and as such involved in oncogenesis. |
|---|
| Immunogen | Protein fraction isolated from detergent extracts of MDCK cells1. |
|---|
| Other Names | Rho guanine nucleotide exchange factor 2 (ARHGEF2), Microtubule-regulated Rho-GEF, Proliferating cell nucleolar antigen p40; KIAA0651; LFP40 |
|---|
| Gene, Accession # | n/a |
|---|
| Catalog # | HM2152 |
|---|
| Price | |
|---|
| Order / More Info | GEF-H1, Human Antibody from HYCULT BIOTECH |
|---|
| Product Specific References | n/a |
|---|
| 产品资料 |
|
|