产品名称 Mph1103I (AvaIII) from UNITED STATES BIOLOGICAL
产品货号 M4680
产品价格 现货询价,电话:010-67529703
产品规格 1000U, 5000U
产品品牌 UNITED STATES BIOLOGICAL
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Product NameMph1103I (AvaIII)
DescriptionSequence: 5'-A T G C A^T-3' 3'-T^A C G T A-5' Source: Moraxella phenylpyruvica RFL1103 Concentration: 10u/ul Unit Definition: One unit is defined as the amount of Mph1103I required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer. Supplied with: R1625: Restriction Enzyme Buffer A, 10X: Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA. R1625-04: Restriction Enzyme Buffer, 10X: Dilute to 1X for use. 1X buffer composition is 10mM Tris-HCl, pH 8.5, 10mM MgCl2, 100mM potassium chloride,, 0.1mg/ml BSA Storage Buffer: Supplied as a liquid in 10mM Tris-HCl (pH 7.4 at 25°C), 200mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA, 50% glycerol. Enzyme Properties: Stability during Prolonged Incubation: A minimum of 0.3 units of Mph1103I is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C. Methylation Effects: Dam/Dcm/CpG/EcoKl: never overlaps- no effect EcoBl: may overlap-effect n
Size1000U, 5000U
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Catalog #M4680
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