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| Product Name | Ubiquitin Conjugating Enzyme E2 D1, N-terminal His-Tag, Recombinant, Human |
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| Description | Purified ≥80%. Source: Recombinant corresponding to aa2-end of human UbcH5a with N-terminal HIS tag expressed in E. coli (NM_003338). Specific Activity: 50ul reaction mix (4mM ATP, 5mM MgCl2, 1mM DTT, UBE1 (0.5ug), UbcH5a or other E2, Biotin-Ubiquitin (0.5ug) and Smurf2 (2) in 50mM Tris pH 7.4 for 30 min at 37°C, then add to streptavidin coated plates for 30 min at 37°C. Incubate with anti-Smurf2, then Europium-labeled secondary antibody, and detect by time resolved fluorescence (lambda-ex=330nm, lambda-em=620nm). Applications: Suitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling. |
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| Size | 100ug |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | Ubiquitin Conjugating Enzyme E2 D1, N-terminal His-Tag, Recombinant, Human (E2(17)KB1, SFT, Stimulator of Fe transport, UBC4/5, UBC4/5 homolog, UbcH5, UBCH5, UbcH5A, UBCH5A, Ubiquitin carrier protein D1, Ubiquitin-conjugating enzyme E2(17)KB 1, Ubiquitin-conjugating enzyme E2-17kD 1, Ubiquitin-protein ligase D1) |
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| Gene, Accession, CAS # | Accession: NM_003338 |
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| Catalog # | U1000-86N |
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| Order / More Info | Ubiquitin Conjugating Enzyme E2 D1, N-terminal His-Tag, Recombinant, Human from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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