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| Product Name | Glutathione Reductase Activity Kit |
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| Description | The Glutathione Reductase Activity kit is designed to quantitatively measure glutathione reductase (GR) activity in a variety of samples. A GR standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. The kit utilizes a proprietary non-fluorescent molecule that will covalently bind to the free thiol group on GSH generated in the reduction of oxidized glutathione (GSSG) to yield a highly fluorescent product. After mixing the sample or standard with StressXpress® Detection Reagent and incubating at room temperature, the fluorescent product is read at 510 nm in a fluorescent plate reader with excitation at 390 nm. Background thiol content is read first after 5 minutes, followed by addition of GSSG and NADPH which uses the standard or sample GR to convert the oxidized glutathione, GSSG, into free GSH, which then reacts with the StressXpress® Detection Reagent to yield the signal related to GR activity The activity of GR in the sample is calculated from the generated signal. We have provided a 96 well plate for measurement but this assay is adaptable for higher density plate formats. The end user should ensure that their HTS black plate is suitable for use with these reagents prior to running samples. Direct Enzyme Activity Assay. Species Reactivity: human. Intra Assay Precision: Five native samples diluted in Assay Buffer and run in replicates of 16 in an assay. The mean and precision of the calculated GR activities were: Sample 1- 3.35 mU/mL, 2.7% CV Sample 2- 2.38 mU/mL, 5.6% CV Sample 3- 1.75 mU/mL, 3.9% CV Sample 4- 0.56 mU/mL, 3.7% CV Sample 5- 0.27 mU/mL, 3.6% CV Inter Assay Precision: Five native samples were diluted in Assay Buffer and run in duplicates in twenty-two assays run over multiple days by four operators. The mean and precision of the calculated GR activities were: Sample 1- 3.35 mU/mL, 5.0% CV Sample 2- 2.36 mU/mL, 12.6% CV Sample 3- 1.64 mU/mL, 6.8% CV Sample 4- 0.62 mU/mL, 10.67% CV Sample 5- 0.27 mU/mL, 10.5% CV. Range: 0.156 - 5 mU/ml. Sensitivity: 0.009 mU/ml. Time: 20 minutes. |
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| Size | 96 well |
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| Concentration | n/a |
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| Applications | Fluorometric assay used to determine glutathione reductase activity by the amount of GSH generated from the reduction of GSSG, in samples. RUO |
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| Other Names | StressXpress® γ-L-Glutamyl-L-cysteinylglycine Activity Kit(2S)-2-Amino-5-[[(2R)-1-(carboxymethylamino)-1-oxo- 3-sulfanylpropan-2-yl]amino]-5-oxopentanoic acid Activity Kit |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | SKT-204-96 |
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| Order / More Info | Glutathione Reductase Activity Kit from STRESSMARQ BIOSCIENCES INC. |
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| Product Specific References | n/a |
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