| 产品详情 |
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| Product Name | RNA Polymerase, T7 |
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| Description | Storage Buffer: 20mM Potassium Phosphate (pH 7.9), 1mM DTT, 0.1mM EDTA, 100mM NaCl and 50% glycerol. Reaction Buffer (5X): 200mM Tris-HCl (pH 7.9 at 25°C), 30mM MgCl, 50mM NaCl, 10mM spermidine. Unit Definition: One unit is the amount of enzyme that incorporates I nmole of [3H] GMP into acid-insoluble fraction in 1 hour at 37°C and pH 8. Purity: Nuclease activity was not detected in any of the following cases, as judged from the intact gel electrophoresis: 1. After incubation of 1ug of λ DNA-Hind III fragments with 500 units of enzyme for 24 hours at 37°C 2. After incubation of 1ug of pBR322 DNA with 500 units of enzyme for 24 hours at 37°C 3. After incubation of 1ug of 16S and 23S rRNA with 500 units of enzyme for 24 hours at 37°C This enzyme was more than 95% homogeneous as judged from SDS-polyacrylamide gel electrophoresis. |
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| Size | 5000U |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | R2049-05 |
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| Price | |
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| Order / More Info | RNA Polymerase, T7 from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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