| 产品详情 |
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| Product Name | Express CRISPR sgRNA Synthesis Kit, BioGenomics™ |
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| Description | Intended Use: Generating sgRNAs for spCas9 system. Test Principle: Express CRISPR sgRNA Synthesis Kit (S. pyogenes) is a neat handy tool for generating sgRNAs for spCas9 system. The 2X Express sgRNA Synthesis SuperMix contains all the components required for the in vitro transcription including an oligo template of the tracer sgRNA. To set up the reaction, simply combine the 2X Express sgRNA Synthesis SuperMix, OneScribe Enzyme Mix, DNA Polymerase, the target-specific oligo, and water in a 20ul reaction. The target-specific oligo first anneals with the sgRNA scaffold complementary oligo included in the 2X Express sgRNA Synthesis SuperMix, and DNA extension will be performed by the DNA polymerase. The newly extended dsDNA will then serve as the template for sgRNA transcription. High quality sgRNA can be obtained in as little as 30 minutes. The synthesized sgRNA can be used directly in downstream applications such as sgRNA in vitro screening. An optional DNase I treatment followed by spi |
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| Size | 25 tests |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 500569 |
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| Order / More Info | Express CRISPR sgRNA Synthesis Kit, BioGenomics™ from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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