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| Product Name | SMURF2, N-terminal FLAG-Tag, Recombinant, Human |
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| Description | Purified ≥80%. Source: Recombinant corresponding to aa130-end of human SMURF 2 with N-terminal FLAG tag expressed in a Baculovirus infected Sf9 cell expression system (NM_022739). Specific Activity: 0.00049 pmol/min/ug. Assay conditions: 50ul reaction mix (4mM ATP, 5mM MgCl2, 1mM DTT, E1 (0.5ug), E2 (2.8ug), Biotin-Ubiquitin (0.5) and Smurf2 (0-2ug) in 50mM Tris pH 7.4) incubate for 30min at 37°C, then add to streptavidin coated plates and incubate for 30min at 37°C. Add antibody against Smurf2, incubate 1 hour. Finally, incubate 1 hour with the Europeum-labeled secondary antibody. Time resolved fluorescence measured at RT (lambda-exc=330 nm, lambda-em=620 nm). Applications: Suitable for use in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors and selectivity profiling. |
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| Size | 20ug |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | SMURF2, N-terminal FLAG-Tag, Recombinant, Human (Smad Ubiquitination Regulatory Factor 2, E3 Ubiquitin-Protein Ligase SMURF2, Smad-Specific E3 Ubiquitin Ligase 2) |
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| Gene, Accession, CAS # | Accession: NM_022739 |
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| Catalog # | S1014-65J |
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| Order / More Info | SMURF2, N-terminal FLAG-Tag, Recombinant, Human from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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