产品名称 Nickel BioAssay™ Kit from UNITED STATES BIOLOGICAL
产品货号 N2545
产品价格 现货询价,电话:010-67529703
产品规格 100 tests
产品品牌 UNITED STATES BIOLOGICAL
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Product NameNickel BioAssay™ Kit
DescriptionNickel is one of four ferromagnetic elements (symbol Ni, at. Num. 28). Several enzymes depend on nickel for activity, including some ureases, carbon monoxide dehydrogenases (methane forming enzymes which reduce CO2 to CH4) and some hydrogenases which allow the production or removal of H2. Most of these activities are found in the archaebacteria. Nickel forms complexes with sulfhydryl compounds with significant absorbance in the UV/visible region in the presence of other ions. The Nickel Assay kit provides a simple method of quantifying Ni+2 in a variety of samples. The assay takes advantage of reaction of Ni+2 with mercaptoethanol in borate buffer to form a complex with strong absorbance bands from ~300 to 600nm. Fe+2 and Co+2 interfere with the assay, therefore extra steps (as described in protocol) must be taken to subtract the interference in order to determine the correct Nickel concentration in mixed samples. Other ions tested (Mn+2, Cu+2, Zn+2) do not interfere with the assay, pr
Size100 tests
Concentrationn/a
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Catalog #N2545
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