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| Product Name | Granulocyte Macrophage Colony Stimulating Factor, Human, BioAssay™ ELISpot Kit |
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| Description | Intended Use: This 2.5 hour ELISpot kit is developed to detect and visualize of single cells secreting human GM-CSF Principle: Add stimulated cells or cells and stimulant to the wells and incubate at 37°C in CO2 incubator for a specified period. Secreted human GM-CSF binds to antibody coated ELISpot plate. ELISpot plate coated with human GM-CSF Capture Antibody. Cells and unbounded proteins are washed away. Streptavidin-AP or Streptavidin-HRP is added and binds to the biotinylated detection antibody. Unbounded proteins are washed away. Biotinylated detection antibody is added and binds to the secreted human GM-CSF. Substrate Solution is added. A colored precipitate forms and appears as spots at the sites of human GM-CSF secreting location. Each individual spot representing an individual human GM-CSF secreting cell. Background: Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) is a member of the hematopoietic cytokine family, which includes interleukin-3 (IL-3) and interleuk |
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| Size | 96 tests |
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| Concentration | n/a |
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| Applications | ELISA |
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| Other Names | Granulocyte Macrophage Colony Stimulating Factor, Human (GM-CSF, Burst Promoting Activity, CMCSF, Colony Stimulating Factor 2, CSF Alpha, Eosinophil Colony Stimulating Factor, Granulocyte Macrophage Colony Stimulating Factor, Molgramostin, Pluripoietin Alpha, Sargramostim), BioAssay™ ELISpot Kit |
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| Gene, Accession, CAS # | Accession: NP_034099 |
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| Catalog # | G8951-17 |
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| Order / More Info | Granulocyte Macrophage Colony Stimulating Factor, Human, BioAssay™ ELISpot Kit from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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