| 产品详情 |
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| Product Name | Biotin-14-CTP |
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| Description | Purity ≥ 90% by reverse phase HPLC. Biotin-14-CTP is a CTP analog that contains biotin attached at the N4-position via a 14-atom linker. The amount of material provided is sufficient for up to 20 transcription reactions (1ug template) using T7 RNA polymerase. Biotin-labeled RNA can be synthesized by SP6, T3, or T7 RNA polymerase and the appropriate template in the presence of ATP, GTP, UTP, and biotin- 14-CTP. Using a 1:1 mixture of biotin-14-CTP:CTP (keeping the total concentration the same as the other nucleotides) will result in a high yield of full length biotin-labeled RNA. Changing the ratio of biotin-14-CTP:CTP to 4:1 (increasing the total concentration of the biotin-14-CTP:CTP mixture to 2.5 times the concentration of each of the other nucleotides) will increase the amount of biotinylation, resulting in the generation of more sensitive probe. Use of the higher biotin-14-CTP:CTP ratio will, however, result in a lower total yield. The biotin-labeled RNA can be detected colorimetr |
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| Size | 500nmol |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | B1750-60 |
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| Price | |
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| Order / More Info | Biotin-14-CTP from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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