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| Product Name | JNK Kinase Activity Screening BioAssay™ Kit |
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| Description | c-Jun N-terminal kinase (JNK) is one of the several main MAP kinase groups identified in mammals. Recent evidences suggest that activation of JNK may play an important role in neuronal apoptosis and other physiological and pathological processes. Sample Type: Cell and tissue lysates. Intended Use: The kit can be used to screen JNK activity relatively quickly for large numbers of samples. Test Principle: The JNK Activity screening BioAssay™ kit utilizes an N-terminal c-Jun (1-79) fusion protein bound to glutathion sepharose beads to selectively ``pull down'' JNK from cell lysates. After wash to remove nonspecifically bound proteins, the kinase reaction is then carried out in the presence of cold ATP. c-Jun phosphorylation is then measured by Western blot analysis using a phospho-c-Jun specific antibody. Kit Components: Kinase Extraction Buffer, 1x80ml c-Jun (1-79) Fusion Protein Beads, 1x800ul Kinase Assay Buffer, 1x25ml ATP, 10mM, 1x50ul Phospho-cJun Specific Antibody, 1x50ul |
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| Size | 40 tests |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 167895 |
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| Order / More Info | JNK Kinase Activity Screening BioAssay™ Kit from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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