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| Product Name | Glutamate Oxidase Assay Kit (red fluorescence) |
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| Description | Amplite™ Fluorimetric Glutamate Oxidase Assay Kit *Red Fluorescence*. Glutamate oxidase belongs to the family of oxidoreductases, specifically those acting on the CH-NH2 group of donors with oxygen as an acceptor. It is an enzyme that specifically catalyzes the oxidative deamination of L-glutamate in the presence of water and oxygen with the formation of o-ketoglutarate, ammonia, and hydrogen peroxide. The Amplite™ Fluorimetric Glutamate Oxidase Assay Kit provides a quick and ultrasensitive method for the measurement of glutamate oxidase in solution and in cell lysates. In the assay, L-glutamic acid is oxidized to ?-ketoglutarate, NH3 and H2O2 by glutamate oxidase. L-Alanine and L-glutamate-pyruvate transaminase are included in the reaction, resulting in multiple cycles of the initial reaction, thus significantly amplifying the production of H2O2. The kit uses our Amplite™ Red substrate which enables a dual recordable mode. The fluorescent signal can be easily read by either a fluorescence microplate reader at Ex/Em = 540/590 nm or an absorbance microplate reader at ~576 nm. With the Amplite™ Glutamate Oxidase Assay kit, we have detected as little as 40 ?U/mL glutamate oxidase in a 100 µL reaction volume. It can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. |
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| Size | 200 tests |
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| Concentration | n/a |
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| Applications | Biossay, Fluorometric |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 11302 |
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| Order / More Info | Glutamate Oxidase Assay Kit (red fluorescence) from AAT BIOQUEST, INC. |
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| Product Specific References | n/a |
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