| 产品详情 |
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| Product Name | SNL Feeder Cells |
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| Description | • Immortalized cell line derived from mouse fibroblast STO cell transformed with murine LIF and neomycin resistance genes• Useful for culture of human or mouse iPS cells as well as ES cell growth• Must be mitotically inactivated prior to addition of stem cellsThe SNL 76/7 cell line, established by Dr. Allan Bradley, is clonally derived from a mouse fibroblast STO cell line transformed with neomycin resistance and murine LIF genes. SNL can be used as |
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| Size | 1 Vial |
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| Concentration | 1 mL, 3 x 10^6 cells/mL in 70% DMEM, 20% FBS, 10% DMSO |
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| Applications | SNL feeder cells are used for the maintenance of ES or iPS cells in the undifferentiated state. The cells must be mitotically inactivated prior to the addition of ES or iPS cells, such as treatment with mitomycin C (2-4 hr, 10 ug/mL). |
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| Other Names | [SNL Feeder Cells] |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | MBS169018 |
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| Price | |
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| Order / More Info | SNL Feeder Cells from MYBIOSOURCE INC. |
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| Product Specific References | 1. McMahon, A.P. and Bradley, A. (1990) Cell 62:1073-1085. 2. Okita, K; Ichisaka, T; Yamanaka, S. (2007) Nature 448:313-317. 3. Takahashi K, Okita K, Nakagawa M, Yamanaka S. (2007) Nat Protoc. 2:3081-9. 4. Takahashi K, Narita M, Yokura M, Ichisaka T, Yamanaka S. (2009) PLoS One 4(12):e8067. 1. Yi, L. et al. (2012). Multiple Roles of p53-Related Pathways in Somatic Cell Reprogramming and Stem Cell Differentiation. Cancer Res. 72: 5635-5645. |
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