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| Product Name | Choline Kinase (ChoK) Activity Kit (Fluorometric) |
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| Description | Pyruvate kinase (PK, EC 2.7.1.40) is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. Lack of pyruvate kinase will slow down the process of glycolysis which causes the disease known as pyruvate kinase deficiency. provides a simple, direct and automation-ready procedure for measuring pyruvate kinase activity in various biological samples such as blood, tissues, and culture cells, etc. In the assay, PEP and ADP were catalyzed by PK to generate pyruvate and ATP. The generated pyruvate is oxidized by pyruvate oxidase to produce color (at ? = 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Since the increase in color or fluorescence intensity is proportional to the increase in pyruvate amount, the PK activity can be accurately measured. The kit detects 0.1 mU/ml pyruvate kinase. Sample types: cell lysates, kidney, liver, purified protein, tissue lysate |
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| Size | n/a |
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| Concentration | n/a |
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| Applications | Measurement of choline kinase activity in different cell lines and tissues |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | LS-K513 |
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| Order / More Info | Choline Kinase (ChoK) Activity Kit (Fluorometric) from LIFESPAN BIOSCIENCES INC. |
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| Product Specific References | n/a |
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