| 产品详情 |
| Edit |   |
|---|
| Product Name | Peroxidase (HRP) Antibody Conjugation Kit (for labeling 25 ug protein) |
|---|
| Description | Buccutite™ Peroxidase (HRP) Antibody Conjugation Kit *Optimized for Labeling 25 ug Protein*. Protein-protein conjugations are commonly performed with a bifunctional linker (such as the commonly used SMCC), having different reactivity on each end for linking two different proteins. One end of the crosslinker reacts (via NHS ester) with amines (-NH2) found in the amino acid lysine and N-terminus, and the other end reacts (via maleimide) with the thiol groups (-SH) found in the amino acid cysteine. However, SMCC-modified protein is extremely unstable and often self-reactive since proteins often contain both amine and thiol groups that cause significant amount of homo-crosslinking. In addition it is quite difficult and tedious to quantify the number of maleimide groups on a protein. Buccutite™ Peroxidase (HRP) Antibody Conjugation Kit is designed for preparing horseradish peroxidase (HRP) conjugates directly from proteins, peptides, and other ligands that contain a free amino group. The HRP provided in our kit has been pre-activated with our proprietary linker Buccutite™ FOL, and can be directly used for conjugation. The entire process only requires two simple mixings without further purification required. The Buccutite™ FOL-activated HRP readily reacts with Buccutite™ MTA-containing molecules under extremely mild neutral conditions without any catalyst required. Compared to commonly used SMCC and other similar technologies, our Buccutite™ bioconjugation system is much more robust and easier to use. It enables faster and quantitative conjugation of biomolecules with higher efficiencies and yields. |
|---|
| Size | 2 labelings |
|---|
| Concentration | n/a |
|---|
| Applications | Biossay |
|---|
| Other Names | n/a |
|---|
| Gene, Accession, CAS # | n/a |
|---|
| Catalog # | 5505 |
|---|
| Price | |
|---|
| Order / More Info | Peroxidase (HRP) Antibody Conjugation Kit (for labeling 25 ug protein) from AAT BIOQUEST, INC. |
|---|
| Product Specific References | n/a |
|---|
| 产品资料 |
|
|