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| Product Name | p-Nitrophenyl Phosphate (pNPP, KR-pT-IRR), Phosphatase Assay Kit, BioAssay™ |
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| Description | Para-nitrophenyl phosphate (pNPP) is a chromogenic substrate for most phosphatases such as alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases. The reaction yields para-nitrophenol, which becomes an intense yellow soluble product under alkaline conditions and can be conveniently measured at 405 nm on a spectrophotometer. Key Features: High sensitivity and wide linear range. The detection limit is generally 3ng phosphatase or below. Homogeneous and simple procedure. No wash or reagent transfer steps are involved. The assay can be completed within 30 minutes. Robust and amenable to HTS. All reagents are compatible with highthroughput liquid handling instruments. Applications: Enzyme Activity Assay and Quality Control for phosphatase production. Characterization of Kinetics of phosphatase reaction. Drug Discovery: high-throughput screen for phosphatase inhibitors. Kit Contents: Catalog # Size (assays) Reagent Assay Buffer Stop Solution |
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| Size | 500 tests, 1000 tests |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | N2600-37 |
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| Order / More Info | p-Nitrophenyl Phosphate (pNPP, KR-pT-IRR), Phosphatase Assay Kit, BioAssay™ from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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