| 产品详情 |
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| Antigenic Specificity | Bromodeoxyuridine/BrdU |
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| Clone | Bu20a |
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| Host Species | Mouse |
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| Reactive Species | n/a |
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| Isotype | IgG1 |
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| Format | Protein G purified |
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| Size | 0.1 mg |
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| Concentration | 1.0 mg/ml |
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| Applications | Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen. For IHC-Frozen: Frozen sections and cell preparations: Acetone-fixed, frozen sections and cell preparations are suitable for staining with clone Bu20a. To survey brain structure overall, use AChE histochemistry, a standard way to identify brain subdivisions. IHC- Formalin-fixed paraffin-embedded tissue sections: Clone Bu20a can be used for labeling paraffin-embedded tissue sections fixed in formalin. Denaturation of the DNA is critical for successful staining of BrdU. This can be achieved by exposing cells to heat, or acid. For heat-induced epitope retrieval, 10mM citrate buffer pH6.0 is recommended. Alternatively, a 30 min incubation in 2M HCl can be performed. The HCl must then be neutralized for 2 min with 0.1 M Na2B4O7. Pretreatment of tissues with proteinase K should be avoided. Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | The Bromodeoxyuridine/BrdU Antibody (Bu20a) from Novus Biologicals is a mouse monoclonal antibody to Bromodeoxyuridine/BrdU. This antibody reacts with non-species specific. The Bromodeoxyuridine/BrdU Antibody (Bu20a) has been validated for the following applications: Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen. Specificity: NB100-64345 recognizes the thymidine analogue bromodoxyuridine (BrdU), which can be incorporated into DNA during S-phase of the cell cycle |
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| Immunogen | Bromodeoxyuridine conjugated to BSA. |
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| Other Names | Bromodeoxyuridine, BUdr |
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| Gene, Accession # | n/a |
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| Catalog # | NB100-64345 |
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| Price | |
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| Order / More Info | Bromodeoxyuridine/BrdU Antibody from NOVUS BIOLOGICALS, LLC |
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| Product Specific References | PubMed: 20445055, 20864654, 2476478 |
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