| 产品详情 |
| Edit |   |
|---|
| Product Name | Phosphodiesterase 1 |
|---|
| Description | Venom exonuclease (Phosphodiesterase I) successively hydrolyzes 5'-mononucleotides from 3'-hydroxy-terminated ribo-and deoxyribo-oligonucleotides. The enzyme has been widely utilized as a tool for structural and sequence studies of nucleic acids. The enzyme has been purified with endonuclease activity being eliminated as well as 5'-nucleotidase and nonspecific monophosphatase. It is nonspecific with respect to base or sugar moieties of nucleotides. A variety of synthetic substrates are hydrolyzed. The exonuclease will not recognize nucleoside units in the syn conformation. ADP-ribosylated proteins are cleaved at the pyrophosphate linkages by venom phosphodiesterase to yield phosphoribosyl-AMP. Activity: ≥20 units per mg dry weight Absorbance (A280): As reported Unit Definition: 1 unit hydrolyzes 1 umole of p-nitrophenyl thymidine-5-phosphate per minute at 25°C, pH 8.9. Nucleotidase: ≤8% Optimum pH: 9.8-10.4 Inhibitors: Reducing agents such as glutathione, cysteine and ascorbic acid. It |
|---|
| Size | 100U |
|---|
| Concentration | n/a |
|---|
| Applications | n/a |
|---|
| Other Names | EC=3.1.4.1; Crotalus adamanteus venom |
|---|
| Gene, Accession, CAS # | n/a |
|---|
| Catalog # | P4072 |
|---|
| Price | |
|---|
| Order / More Info | Phosphodiesterase 1 from UNITED STATES BIOLOGICAL |
|---|
| Product Specific References | n/a |
|---|
| 产品资料 |
|
|