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| Product Name | Phosphodiesterase 4A1A, Recombinant, Human, GST-Tag (PDE4A1A) |
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| Description | Purity >70% by SDS-PAGE. Human PDE4A1A (GenBank Accession No. U97584), full length protein with N-terminal GST tag (MW = 100kD), expressed in a Baculovirus infected Sf9 cell expression system. Specific Activity: Lot Specific. Sample Lot Value: 4,176 pmol/min/ug. Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3?, 5?-cAMP to 5?-AMP per min at 37°C. Assay Conditions: 10mM Tris-HCl, pH 7.4, 10mM MgCl2, 1mM MnCl2, 200uM cAMP, and 0.2ng/ul PDE4A1A at 37°C for 20 min. Quantified by 5?-nucleotidase cleaving the 5?-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
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| Size | 10ug |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | Accession: U97584 |
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| Catalog # | P4072-03A |
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| Order / More Info | Phosphodiesterase 4A1A, Recombinant, Human, GST-Tag (PDE4A1A) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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