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| Product Name | Caspase-4 Colorimetric BioAssay™ Kit |
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| Description | Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-4 Colorimetric BioAssay™ Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEVD. Sample Type: Cell and tissue lysates Intended Use: For use in detecting the early/middle stages of apoptosis and differentiating apoptosis from necrosis. Specificity: Recognizes Caspase-4. Species Crossreactivity: mammalian Test Principle: The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate LEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in LEVD-dependent Caspase activity. Kit Components: Cell Lysis Buffer, 1x100ml?2X Reaction Buffer, 1x32ml?LEVD-pNA, 4mM, 2x1ml?DTT, |
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| Size | 400 tests |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 219566 |
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| Order / More Info | Caspase-4 Colorimetric BioAssay™ Kit from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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