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| Product Name | Caspase 8 Colorimetric Substrate, N-acetyl-Asp-Glu-Val-Asp-pNA p-nitroanilide (FLICE, MASH, Mch5) |
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| Description | Purity ≥ 95% (HPLC). Colorimetric substrate for Caspase-8. Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. See Caspase-8 Apoptosis Detection Kit C2087-40 which provides a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on detection of cleavage of substrate IETD-pNA (p-nitroanilide). Upon proteolytic cleavage of the substrate by caspases, free pNA is detected at a wavelength of 400nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the increase in Caspase-3 activity. FLICE and related caspase activity can be quantified by spectrophotometric detection of free pNA (lmax 400nm) after cleavage from the peptide substrate IETD-pNA, using a spectrophotometer or multi-well plate reader. Peptide sequence: Ac-Ile-Glu-Thr-Asp-pNA |
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| Size | 200 tests, 1000 tests |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | C2087-41 |
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| Order / More Info | Caspase 8 Colorimetric Substrate, N-acetyl-Asp-Glu-Val-Asp-pNA p-nitroanilide (FLICE, MASH, Mch5) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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