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| Product Name | Caspase 7 Immunoassay Kit, BioAssay™ (Mch-3, ICE-LAP3, CMH-1) |
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| Description | Caspases play a key role in initiation of cellular events during apoptosis. The Caspase-7 Immunoassay Kit provides an effective immunosorbent enzyme assay for the specific, quantitative detection of caspase-7 activity in microtiter plates. The assay utilizes caspase-7 polyclonal antibody to capture activated caspase-7 from cell lysates. Substrate DEVD-AFC is then added that is cleaved proportionally to the amount of activated caspase-7 in the cell lysate. The cleavage generates free AFC which is then analyzed fluorometrically (Ex 400nm and Em 505nm) using a fluorescence plate reader. The assay ensures absolute specific detection of caspase-7. Other known caspases and non-specific proteases are not detected. Kit Components: • Cell Lysis Buffer • Coating Buffer • Anti-Caspase-7 Antibody (20X) • Blocking Buffer • Incubation Buffer • DTT (1M) • DEVD-AFC Substrate (1mM) • Positive Control (rh-Caspase-7) • Microtiter Plate • Adhesive Plate Co |
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| Size | 100 tests |
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| Concentration | n/a |
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| Applications | ELISA |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | C2087-29B |
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| Order / More Info | Caspase 7 Immunoassay Kit, BioAssay™ (Mch-3, ICE-LAP3, CMH-1) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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