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| Product Name | Caspase 6 (McH2), Apoptosis Detection, Colorimetric BioAssay™ Kit |
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| Description | Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Mch2/Caspase-6 Colorimetric Protease Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence VEID. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate VEID-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in Mch2/caspase-6 activity. Components: 25 assays 100 assays Cell Lysis Buffer 25ml 100ml 2X Reaction Buffer 2ml 4 x 2ml VEID-pNA Substrate (4 mM) 125ul 500ul DTT (1M) 100ul 400ul Dilution Buffer 25 ml 100 ml Stability: Store kit at -20°C (Store Cell Lysis Buffer, Dilution Buffer and Reaction Buffer (2X) at 4°C after opening). All reagents are stable for 1 ye |
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| Size | 25 tests, 100 tests, 200 tests |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | C2087-25 |
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| Order / More Info | Caspase 6 (McH2), Apoptosis Detection, Colorimetric BioAssay™ Kit from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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