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| Product Name | Caspase 6 (McH2), Apoptosis Detection, Fluorometric BioAssay™ Kit |
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| Description | Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Mch2/Caspase-6 Fluorometric Protease Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence VEID. The assay is based on detection of cleavage of substrate VEID-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). VEID-AFC emits blue light (lmax 400nm); upon cleavage of the substrate by Mch2 or related caspases, free AFC emits a yellow-green fluorescence (lmax 505nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in Mch2 activity. Components: 25 assays 100 assays Cell Lysis Buffer 25ml 100ml 2X Reaction Buffer 2ml 4 x 2ml VEID-AFC Substrate (4mM) 125ul 500ul DTT (1M) 100ul 400ul Stability: Store kit at -20°C (Store Cell Lysis Buffer and 2X Reaction Buffer at 4°C after ope |
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| Size | 25 tests, 100 tests, 200 tests |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | C2087-26 |
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| Order / More Info | Caspase 6 (McH2), Apoptosis Detection, Fluorometric BioAssay™ Kit from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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