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| Product Name | Phospholipase D Assay Kit, BioAssay™ |
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| Description | Phospholipase D (PLD) catalyses the hydrolysis of the phosphodiester bond of glycerophospholipids to generate phosphatidic acid and a free headgroup. Abnormalities in PLD expression have been associated with human cancers. This method provides a simple and high-throughput assay for measuring PLD activity. In this assay, PLD hydrolyzes phosphatidylcholine to choline which is determined using choline oxidase and a H2O2 specific dye. The optical density of the pink colored product at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the PLD activity in the sample. Key Features: Sensitive. Use 10ul samples. Detection range: Colorimetric Assay: 0.06-10 U/L, Fluorometric Assay: 0.04-1 U/L. Simple and High-throughput: the assay involves addition of a single working reagent and can be readily adapted to high-throughput assays for drug screening. Applications: Direct Assays: phospholipase D in biological samples. Drug Discovery/Pharmacology: effects of drugs on phospholip |
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| Size | 96 tests |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | P4074-50P |
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| Order / More Info | Phospholipase D Assay Kit, BioAssay™ from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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