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| Product Name | Histone H3, phosphorylated (Ser10) Sandwich ELISA Kit, BioAssay™ |
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| Description | Detects endogenous levels of Phospho-Histone H3 (Ser10). Using this Sandwich ELISA Kit Phospho- Histone H3 (Ser10) is detected when treated with Calyculin A in NIH 3T3 cells. However, the levels of Histone H3 remains unchanged, as shown by Western analysis using Histone H3. Phospho-Histone H3 (Ser10) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Phospho-Histone H3 (Ser10) protein. A Histone H3 Antibody has been coated onto the microwells. After incubation with cell lysates, both nonphospho- and phospho-Histone H3 proteins are captured by the coated antibody. Following extensive washing, a Biotinylated Phospho-Histone H3 (Ser10) Antibody is added to detect the captured phospho-Histone H3 protein. HRP-linked Streptavidin is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of optical density for this developed color is proportional to the quantity of |
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| Size | 1 kit |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | H5110-12N |
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| Order / More Info | Histone H3, phosphorylated (Ser10) Sandwich ELISA Kit, BioAssay™ from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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