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| Product Name | Sepharose, Metal-chelating |
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| Description | Metal-chelating Sepharose consists of iminodiacetic acid groups coupled to Sepharose. It is supplied free of metal ions and has to be charged with a suitable metal ion before use. It is not always possible to predict which metal ion is most appropriate. The metal ions most frequently used are Ni2+, Cu2+, Zn2+. A single exposed histidine residue may result in adsorption of the protein on Cu2+ while two vicinal histidine residues are needed for adsorption on Zn2+, Ni2+ ions are often used for the purification of polyhistidine-tagged fusion protein. In some cases Cu2+, Co2+, and Ca2+ have been used with success. |
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| Size | 25ml |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | S0945-50 |
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| Order / More Info | Sepharose, Metal-chelating from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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