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Product Name | Recombinant Human AMPK (G1/B2/A2) Heterotrimer Protein |
Description | MW: 38,533 Da. Purity >90 % as determined by SDS-PAGE. A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB2 (O43741) (Met 1-Ile 272), constructed the plasmid 2; A DNA sequence encoding the human PRKAA2 (P54646) (Met 1-Arg 552) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified. |
Size | 0.02 mg, 0.05 mg |
Concentration | n/a |
Applications | The recombinant heterotrimer of human AMPK (PRKAG1 / PRKAB2 / PRKAA2) has a calculated molecular mass of 158 (38+30+90) KDa. The apparent molecular mass is approximately 35, 37 & 95 KDa respectively in SDS-PAGE under reducing conditions. |
Other Names | [AMPK, 5' AMP-activated protein kinase, 5' adenosine monophosphate-activated protein kinase. Heterotrimer of alpha 2 catalytic subunit, beta 2 non-catalytic subunit and gamma 1 non-catalytic subunit.] |
Gene, Accession, CAS # | AMPK, Gene ID: 5571, Accession: NP_001193638.1 |
Catalog # | MBS2545013 |
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Order / More Info | Recombinant Human AMPK (G1/B2/A2) Heterotrimer Protein from MYBIOSOURCE INC. |
Product Specific References | n/a |
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