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| Product Name | Mdh, Recombinant, E.coli, aa1-312, His-Tag(Malate Dehydrogenase) |
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| Description | Purity ~95% (SDS-PAGE). Malate dehydrogenase (mdh) belongs to the LDH/MDH superfamily and MDH type 1 family. This enzyme catalyzes the conversion of malate into oxaloacetate (using NAD+) and vice versa (this is a reversible reaction). Malate dehydrogenase is also involved in gluconeogenesis, the synthesis of glucose from smaller molecules. Source: Recombinant protein corresponding to aa1-312 from E. coli Mdh, fused to His-tag at N-terminal, expressed in E. coli. Molecular Weight: ~34.9kD (336aa) Specific Activity: >1000units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta- NAD/minute at pH 8.0 at 37°C. Activity Assay: 1. Prepare a 180ul assay buffer into a suitable container. The concentrations are 100mM potassium phosphate (pH8.0), 0.5mM beta-nicotinamide adenine dinucleotide, reduced form, 0.5mM oxalacetic acid 2. Equilibrate to 37°C and monitor at A340nm until the value is constant using a spectrophotometer. 3. Add 20ul of recombinant MDH pro |
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| Size | 100ug, 500ug |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | Accession: NP_417703 |
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| Catalog # | 351364 |
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| Order / More Info | Mdh, Recombinant, E.coli, aa1-312, His-Tag(Malate Dehydrogenase) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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