| 产品详情 |
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| Product Name | Bpl I |
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| Description | 5'-^ 8(N) G A G (N)5 C T C (N)13^-3' 3'-^13(N) C T C (N)5 G A G (N) 8^-5' BplI requires only Mg2+ for its activity, but is stimulated by S-adenosylmethionine. 0.05mM S-adenosylmethionine gives more than a 100-fold increase of BplI activity. The cleavage of DNA by BplI is never complete. Concentration: 5 units/ul Unit Definition: One unit is defined as the amount of Bpl I required to digest 1ug of lambda DNA-Xhol in 1 hour at 37°C in 50ul of assay buffer (a maximal cleavage level is achieved at which no change in the fragmentation pattern is observed with further increase of enzyme). Storage Buffer: Supplied as a liquid in 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA, 50% glycerol. Buffers Supplied: R1625 Restriction Enzyme Buffer A, 10X: 33mM Tris-acetate, pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA B2645-01: SAM 0.05mM S-adenosylmethionine |
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| Size | 100U, 500U |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | B2645 |
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| Price | |
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| Order / More Info | Bpl I from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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