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| Product Name | PDE1B Cell-Based Reporter BioAssay™ Kit |
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| Description | PDE1B plays a key role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. PDE1B hydrolyzes both cAMP and cGMP to nucleoside 5?-monophosphate and prefers cGMP at low substrate levels. The PDE1B Cell-Based Reporter BioAssay™ Kit is designed for identification of PDE1B inhibitors in cultured cells. The kit includes a recombinant HEK293 cell line that stably expresses human PDE1B, atrial natriuretic peptide (ANP), mammalian expression constructs for ANP receptor A, CNGA2, and aequorin, and buffer for the assay. In the PDE1B reporter assay, PDE1B-HEK293 cells are transiently transfected with expression vectors for atrial natriuretic peptide (ANP) receptor A (ANPRA, a guanylate cyclase), cyclic nucleotide-gated cation channel CNGA2, and the photoprotein aequorin. When cells are activated with ANP, ANP induces the production of cGMP through ANPRA. cGMP can activate the CNGA2 channel and induces Ca2+ influx, resulting in aequorin luminescence. The |
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| Size | 500 tests |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 171960 |
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| Order / More Info | PDE1B Cell-Based Reporter BioAssay™ Kit from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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