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| Product Name | PDE, BioAssay™ Kit (Phosphodiesterases) |
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| Description | Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. The PDE Assay Kit is designed for identification of PDE inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDEs to the binding agent. Intended Use: Designed for identification of PDE inhibitors using fluorescence polarization. Test Principle: The PDE inhibitor screening assay kit comes in a convenient 96-well format, with fluorescently labeled substrate (cAMP and cGMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the PDE Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE reactions. First, the fluorescently labeled cAMP or cGMP is incubated with a sample containing PDE2A (cAMP-dependent) or PDE5A (cGMP-dependent) for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent |
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| Size | 96 tests |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 137322 |
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| Order / More Info | PDE, BioAssay™ Kit (Phosphodiesterases) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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