| 产品详情 |
| Edit |   |
|---|
| Product Name | ELISA cAMP Assay Kit |
|---|
| Description | Screen Quest™ Fluorimetric ELISA cAMP Assay Kit. Screen Quest™ Fluorescence ELISA cAMP Assay Kit provides an optimized assay method for monitoring the activation of adenylyl cyclase in G-protein coupled receptor systems. The assay is based on the competition for a fixed number of antibody binding sites between HRP-labeled cAMP and non-labeled cAMP. HRP-cAMP is displaced from the HRP-cAMP/anti-cAMP antibody complex by unlabeled free cAMP. In the absence of cAMP, HRP-cAMP conjugate is bound to anti-cAMP antibody exclusively. However, the unlabeled free cAMP in the test sample competes for anti-cAMP antibody with the HRP-cAMP antibody conjugate, therefore inhibits the binding of HRP-cAMP to anti-cAMP antibody. Our Screen Quest™ Fluorometric cAMP Assay Kit provides a sensitive method for detecting adenylate cyclase activity. Compared to other commercial ELISA cAMP assay kits, this cAMP assay kit only requires a single wash step to remove unbound material prior to the development step. It also eliminates the tedious acetylation step. The kit uses Amplite™ Red as a fluorogenic HRP substrate to quantify the HRP activity. The fluorescent product formed is proportional to the activity of HRP-cAMP conjugate. |
|---|
| Size | 10 plates |
|---|
| Concentration | n/a |
|---|
| Applications | Biossay, Fluorometric |
|---|
| Other Names | n/a |
|---|
| Gene, Accession, CAS # | n/a |
|---|
| Catalog # | 36374 |
|---|
| Price | |
|---|
| Order / More Info | ELISA cAMP Assay Kit from AAT BIOQUEST, INC. |
|---|
| Product Specific References | n/a |
|---|
| 产品资料 |
|
|