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| Product Name | Recombinant Pfu DNA Polymerase |
| Description | MW: 90,113 Da. Description: Pfu DNA Polymerase is a thermo-stable enzyme having a Mw of about 90kDa. Pfu DNA Polymerase is derived from E Coli that and cloned from Pyrococcus furiosus strain Vc1 DSM3638. Pfu DNA Polymerase replicates DNA at 75 degree C, catalyzing the polymerization of nucleotides into duplex DNA in the 5 ? to 3 ? direction in the existence of magnesium. Pfu DNA Polymerase possesses 3 ? to 5 ? exonuclease (proofreading) activity. Base misinsertions that take place during |
| Size | 100 units, 500 units, 2500 units |
| Concentration | n/a |
| Applications | 1. Ideal for high-fidelity amplification. 2. 3'-5' exonuclease activity provides a low error rate. 3. One of the most thermostable DNA polymerases known. 4. Lack of extendase activity means no unwanted 3' overhangs. 5. Optimal for blunt-end PCR cloning. 6. Optimum temperature near 75 degree C. 7. 95% active after 1-hour incubation at 98 degree C. |
| Other Names | [Pfu DNA Polymerase; Pfu-DNA Polymerase Recombinant; DNA polymerase; EC 2.7.7.7; Pfu polymerase; Pfu-DNA Polymerase; Pfu DNA Polymerase] |
| Gene, Accession, CAS # | PF0212; pol, Gene ID: 1468044, Accession: NP_577941.1 |
| Catalog # | MBS142406 |
| Price | |
| Order / More Info | Recombinant Pfu DNA Polymerase from MYBIOSOURCE INC. |
| Product Specific References | n/a |