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| Product Name | VD3 (Vitamin D3) BioAssay™ ELISA Kit (Bovine) |
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| Description | Sample Type: Serum, plasma, tissue homogenates and other biological fluids. Intended Use: Competitive ELISA, Coated with Antigen Sensitivity: <1.875ng/ml Range: 3.125-200ng/ml Specificity: Bovine Test Principle: This BioAssay™ ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with VD3. During the reaction, VD3 in the sample or standard competes with a fixed amount of VD3 on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to VD3. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of VD3 in the samples is then determined by comparing the O.D. of the samples |
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| Size | 48 tests, 96 tests |
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| Concentration | n/a |
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| Applications | ELISA |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 370300 |
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| Order / More Info | VD3 (Vitamin D3) BioAssay™ ELISA Kit (Bovine) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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