产品名称 MvaI (EcoRII) from UNITED STATES BIOLOGICAL
产品货号 M9580
产品价格 现货询价,电话:010-67529703
产品规格 2000U
产品品牌 UNITED STATES BIOLOGICAL
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Product NameMvaI (EcoRII)
Description5'-C C^A G G-3' 3'-G G T^C C-5' NOTE: MvaI-neoschizomer of EcoRII, produces DNA fragments that have a 1-base 5'-extension. Unlike EcoRII, MvaI is not blocked by Dcm methylation. Source: E.coli that carries the cloned mvaIR gene from Micrococcus varians RFL19 Concentration: 10u/ul Unit Definition: One unit is defined as the amount of M9580 required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer. Supplied in 10mM Tris-HCl, pH 7.5, 400mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol. Supplied With: R1625: Restriction Enzyme Buffer A, 10X: Supplied as a liquid in 33mM Tris-acetate, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA (pH 7.9 at 37°C). R1625-04: Restriction Enzyme Buffer E, 10X: Supplied as a liquid in 10mM Tris-HCl, pH 8.5, 10mM MgCl2, 100mM KCl and 0.1mg/ml BSA. Incubate at 37°C. Dilute with: 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
Size2000U
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Catalog #M9580
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